杩戞棩锛屽寳浜ぇ瀛﹀寲瀛︿笌鍒嗗瓙宸ョ▼瀛﹂櫌璐炬鑺宠棰樼粍涓庤嫳鍥界害缈拌嫳绾虫柉涓績Caroline Dean璇鹃缁勫悎浣滐紝鍦?/span>Nature Communication鏉傚織鍦ㄧ嚎鍙戣〃棰樹负鈥淩-loop resolution promotes co-transcriptional chromatin silencing鈥?/span>鐨?/span>鐮旂┒璁烘枃銆傛椤圭爺绌舵彮绀轰簡RNA缁撳悎铔嬬櫧FCA鍦?/span>m6A淇グ鐨勬鍙嶉浣滅敤涓嬶紝閫氳繃鍏辫浆褰?/span>RNA鍔犲伐杩囩▼璋冭妭R鐜紙R-Loop锛夌殑绋冲畾鎬?/span>锛屽苟瑙﹀彂鏌撹壊璐ㄦ矇榛樼殑鏂版満鍒?/span>銆?/span>
鍩哄洜濡備綍鍦ㄦ伆褰撶殑鏃堕棿鍜岀粍缁囧櫒瀹樹腑姝ｇ‘琛ㄨ揪鏄幇浠ｇ敓鐗╁鐨勬牳蹇冮棶棰橈紝RNA浠?/span>瀵肩殑鏌撹壊璐ㄦ矇榛樺湪鍏朵腑璧峰埌浜嗗叧閿綔鐢ㄣ�傜劧鑰?/span>鍥?/span>浜庣爺绌舵墜娈碉紝鍒扮洰鍓嶄负姝紝浜轰滑瀵硅繖涓�杩囩▼鍏蜂綋鏈哄埗鐨勪簡瑙ｈ繕寰堟湁闄愩�傚湪妯″紡妞嶇墿鎷熷崡鑺?/span>涓紝鎶戣姳鍩?/span>鍥?/span>FLC鏄爺绌?/span>RNA浠嬪鐨勬煋鑹茶川娌夐粯鐨勭粡鍏告ā鍨嬨�?/span>FLC鍏锋湁涓�绨囧ぉ鐒跺弽涔夎浆褰曟湰锛岀О涓?/span>COOLAIR銆傚湪鏃╁厛鐨勭爺绌朵腑锛屼汉浠彂鐜颁竴绯诲垪RNA缁撳悎铔嬬櫧锛堝FCA锛?/span>FPA锛?/span>FLK绛夛級锛?/span>3鈥?/span>鏈鍔犲伐鍥犲瓙锛堝FY锛?/span>CstFs绛夛級锛?/span>RNA鍓帴鍥犲瓙锛堝PRP8绛夛級閮戒績杩涗簡COOLAIR鐨勮繎绔仛鑵鸿嫹閰稿寲銆傝�屽湪COOLAIR鐨勮繎绔仛鑵鸿嫹閰稿寲杩囩▼涓紝涓�绯诲垪鏌撹壊璐ㄤ慨楗扮浉鍏宠泲鐧斤紙濡?/span>FLD锛?/span>LD锛?/span>SDG26绛夛級琚嫑鍕熻嚦FLC鍩哄洜搴т綅涓婏紝鏀瑰彉浜?/span>FLC鍩哄洜搴т綅涓婃煋鑹茶川鐨勭姸鎬侊紝闄嶄綆浜?/span>FLC鐨勮〃杈撅紝鏈�缁堜績杩涗簡鎷熷崡鑺?/span>鐨勬垚鑺辫浆鍖栥�?/span>
鍙︿竴鏂归潰锛屾湁鐮旂┒鏄剧ずR鐜?/span>涓庢煇浜涙煋鑹茶川娌夐粯鐨勬爣蹇楁�т慨楗扮浉鍏宠仈锛岃�?/span>鍦ㄤ箣鍓嶇殑宸ヤ綔涓紝Caroline Dean璇鹃缁勬浘鍙戠幇鍦?/span>FLC鍩哄洜鐨?/span>3鈥?/span>绔瓨鍦ㄧ潃鐢?/span>COOLAIR杞綍瀵艰嚧鐨?/span>R鐜紝鍗曢摼DNA缁撳悎铔嬬櫧NDX鍙互绋冲畾璇?/span>R鐜殑瀛樺湪锛屽苟褰卞搷FLC鍙?/span>COOLAIR鐨勮〃杈撅紙Sun et al. 2013锛夈�?/span>鐒惰�岃繖涓�R鐜粨鏋勬槸鍚﹀弬涓?/span>RNA浠?/span>瀵肩殑鏌撹壊璐ㄦ矇榛樿繕鏈夊緟杩涗竴姝ユ帰绱€�?/span>
涓烘锛岃鐮旂┒棣栧厛閫氳繃fca涓?/span>ndx绐佸彉浣撶殑閬椾紶浜掍綔锛?/span>鍙戠幇NDX瀵?/span>FLC鍩哄洜鐨勬矇榛樹綔鐢ㄤ緷璧?/span>FCA銆?/span>NDX绋冲畾R鐜粨鏋勫彲浠ヤ績杩?/span>FCA铔嬬櫧涓庢柊鐢?/span>COOLAIR杞綍鏈殑缁撳悎锛屽苟瑙﹀彂鏌撹壊璐ㄤ慨楗扮姸鎬佺殑鏀瑰彉銆傚湪姝よ繃绋嬩腑锛?/span>FCA閫氳繃鎷涘嫙3鈥?/span>鏈鍔犲伐鍥犲瓙FY锛?/span>瀹屾垚COOLAIR鐨勮繎绔仛鑵鸿嫹閰稿寲锛?/span>骞朵績杩?/span>R鐜粨鏋勭殑娑堣В銆?/span>
鏈夎叮鐨勬槸锛屽湪瀵?/span>FCA鐩镐簰浣滅敤铔嬬櫧鐨勯壌瀹氫腑锛屼笉浠呭彂鐜颁簡FY绛?/span>3鈥?/span>鏈鍔犲伐鍥犲瓙锛岃繕鍙戠幇浜?/span>m6A鐢插熀杞Щ閰跺鍚堢墿鐨勭粍鍒?/span>MTA銆?/span>MTB浠ュ強FIP37锛屼笖杩欑鐩镐簰浣滅敤鏄笉渚濊禆RNA銆?/span>閬椾紶鍒嗘瀽鏄剧ず锛?/span>MTA鐨勭己澶变弗閲嶆姂鍒朵簡FCA鐨勫姛鑳斤紝瀵艰嚧FLC鐨?/span>楂樿〃杈?/span>鍙婂紑鑺辩殑鎺ㄨ繜銆?/span>FCA铏界劧涓嶇洿鎺ョ粨鍚?/span>m6A淇グ锛屼絾浣撳唴浣撳瀹為獙鍧囨樉绀猴紝m6A澧炲己浜?/span>FCA瀵?/span>鏂扮敓COOLAIR杞綍鏈?/span>鐨勭粨鍚堬紝骞跺奖鍝嶄簡FCA鏍稿唴鍑濊仛浣?/span>鐩稿垎绂?/span>鐨勫舰鎴?/span>銆?/span>鍚屾椂锛?/span>FCA鍙?/span>閫氳繃鐩稿垎绂讳綔鐢?/span>淇冭繘浜?/span>m6A淇グ鐨勭敓鎴?/span>锛屽舰鎴愪簡涓�涓鍙嶉鐨勫惊鐜�?/span>
杩涗竴姝ョ爺绌惰〃鏄庯紝鍦?/span>m6A淇グ鐨勮皟鑺備笅锛?/span>FCA閫氳繃鍏辫浆褰?/span>RNA鍔犲伐杩囩▼璋冭妭R鐜ǔ瀹氭�э紝瀵逛簬鍏朵綔鐢ㄧ殑鏂扮敓RNA搴曠墿鏄竴涓櫘閬嶇殑鏈哄埗銆傝�?/span>褰撹浆褰曟湰寤朵几鍙楀埌灞�閮ㄦ煋鑹茶川鐜鐨勫奖鍝嶏紝鍑虹幇闈炶鑼冪殑锛?/span>Non-canonical锛夎仛鑵鸿嫹閰稿寲鏃讹紝3鈥?/span>鏈鍔犲伐鍥犲瓙渚夸細鎷涘嫙涓�绯诲垪鏌撹壊璐ㄤ慨楗扮浉鍏宠泲鐧斤紝瀵艰嚧璇ュ熀鍥犲骇浣嶇殑鏌撹壊璐ㄦ矇榛橈紝鎷熷崡鑺ョ殑COOLAIR/FLC渚垮睘浜庤繖涓�鎯呭舰銆?/span>杩欓」宸ヤ綔涓?/span>R鐜粨鏋勶紝RNA鏈鍔犲伐鍥犲瓙锛?/span>m6A淇グ鐨勫姩鎬佺浉浜掍綔鐢ㄥ苟瑙﹀彂鏌撹壊璐ㄦ矇榛樼殑鏈哄埗鎻愪緵浜嗘柊鐨勮瑙ｃ�?/span>
绾︾堪鑻辩撼鏂腑蹇?/span>Caroline Dean璇鹃缁勭殑璁?/span>楠?/span>鐟跺崥澹?/span>銆?/span>鍚存煒鍗氬＋锛堢幇涓哄崡鏂圭鎶�澶у鐢熺墿绯?/span>鐮旂┒鍛?/span>锛?/span>锛屽寳浜ぇ瀛﹀寲瀛︿笌鍒嗗瓙宸ョ▼瀛﹂櫌璐炬鑺宠棰樼粍鐨勬娲秴鍗氬＋涓烘湰鏂囧叡鍚岀涓�浣滆�咃紝璐炬鑺崇爺绌跺憳鍜?/span>Caroline Dean鏁欐巿涓烘湰鏂囧叡鍚岄�氳浣滆�咃紝Caroline Dean璇鹃缁勭殑鏂规檽宄板崥澹紙鐜颁负娓呭崕澶у鐢熷懡绉戝瀛﹂櫌鐮旂┒鍛橈級瀵规湰鏂囦害鏈夎础鐚�?/span>璇ュ伐浣滃緱鍒颁簡鍥藉鑷劧绉戝鍩洪噾濮?/span>銆?/span>涓浗鍗氬＋鍚庣瀛﹀熀閲戜細浠ュ強鍖椾含鍒嗗瓙绉戝鍥藉鐮旂┒涓績鐨勮祫鍔┿�?/span>
鍏ㄦ枃閾炬帴锛?/span>//www.nature.com/articles/s41467-021-22083-6