瀹為獙瀹ゆ柊闂?/h4>
闄堥箯/妯婃柊鍏冨洟闃熸垚鍔熷紑鍙戝熀浜庣敓鐗╂浜ゅ厜鍌寲鐨勭嚎绮掍綋缁勫鏂版妧鏈疌AT-Prox
绾跨矑浣?/span>浣滀负缁嗚優鐨勮兘閲忓伐鍘傚湪澶氫釜淇″彿閫氳矾浠ュ強澶氱鐤剧梾鐨勫彂鐢熷彂灞曚腑鎵紨浜嗙浉褰撻噸瑕佺殑瑙掕壊銆?/span>瀵圭嚎绮掍綋铔嬬櫧璐ㄧ粍杩涜澶氱淮搴︾殑瑙ｆ瀽鑳藉浣挎垜浠?/span>鏇存繁鍏ュ湴浜嗚В瀹冪殑鍔熻兘銆傜劧鑰岋紝鐜版湁鐨勭嚎绮掍綋铔嬬櫧璐ㄧ粍瀛︽妧鏈瓨鍦ㄧ浉搴旂殑缂洪櫡銆傚叾涓紝閫氳繃瀵嗗害姊害绂诲績寰楀埌鐨勭嚎绮掍綋瀹规槗琚叾浠?/span>缁嗚優鍣?/span>姹℃煋锛屼笖瀵瑰垎绂诲悗鐨勭嚎绮掍綋杩涜铔嬬櫧璐ㄧ粍瀛﹀垎鏋愪笉鑳藉鍙嶆槧娲荤粏鑳炲唴鐨勭湡瀹炴儏鍐点�傞叾淇?/span>閭昏繎鏍囪浠ュ強浜氱粏鑳?/span>瀹氫綅鐨勬椿鎬?/span>鍖栧鍒嗗瓙鏍囪鎶�鏈父鐢ㄤ簬娲荤粏鑳炰腑鐨勭嚎绮掍綋铔嬬櫧璐ㄧ粍瀛﹀垎鏋愶紝鐒惰�岃繖涓ょ鎶�鏈?/span>鍒嗗埆鍙楅檺浜庤川绮掕浆鏌撶殑鎿嶄綔闅惧害浠ュ強娲绘�?/span>鍖栧鍒嗗瓙鐨?/span>闈炵壒寮傛�ф爣璁?/span>銆?/span>
鍥?/span>1. CAT-Prox鎶�鏈?/span>绀烘剰鍥?/span>
杩戞棩锛屽寳浜ぇ瀛﹀寲瀛︿笌鍒嗗瓙宸ョ▼瀛﹂櫌闄堥箯/妯婃柊鍏冨洟闃熸姤閬撲簡涓�绉?/span>鍩轰簬鍏夊偓鍖栫殑鐢熺墿姝ｄ氦鍙嶅簲鐨?/span>鏂颁竴浠?/span>绾跨矑浣撹泲鐧借川缁勫鎶�鏈?/span>CAT-Prox锛?/span>Photocatalytic decaging-enabled proximity labeling strategy锛?/span>銆傝鐮旂┒鍒╃敤鍏夊偓鍖栫殑鐢熺墿姝ｄ氦鍙嶅簲鐨勭嫭鐗逛紭鍔匡紝鎻愬嚭鍏夋晱鍓傞澏鍚戠嚎绮掍綋鍜屽彲瑙佸厜澶栨簮鎺у埗鐨勨�滃寲瀛?/span>-鍏夋帶鈥濆崗鍚岀瓥鐣ワ紝鍦ㄦ椿缁嗚優鐨勭嚎绮掍綋涓師浣嶅偓鍖栦骇鐢熸椿鎬у寲瀛︽帰閽堬紝鐢ㄤ簬绾跨矑浣撹泲鐧借川缁勭殑鍘熶綅銆佸疄鏃舵爣璁帮紝瀹炵幇浜嗘棤闇�鍩哄洜鎿嶄綔銆佹椂绌虹伒娲诲彲鎺х殑绾跨矑浣撹泲鐧借川鐨勫師浣嶆爣璁帮紝鍦ㄩ毦浠ュ熀鍥犳搷浣滅殑缁嗚優涓疄鐜颁簡楂樼壒寮傜殑绾跨矑浣撹泲鐧借川缁勫鐨勨�滄椂-绌衡�濆垎鏋愩�?/span>
浣滆�呴鍏堝皢鍝嶅簲钃濆厜鐓у皠鐨?/span>鍏夊偓鍖栧墏瀹氫綅鑷崇嚎绮掍綋锛岄殢鍚?/span>鍚戠粏鑳炰腑鍔犲叆鍙犳爱鑻熀淇濇姢鐨勪簹鐢插熀閱?/span>鍖栧悎鐗?/span>銆傚叾涓紝鍙犳爱鑻熀鑳藉鍦ㄨ摑鍏夌殑鐓у皠涓嬭鍏夊偓鍖栧墏鍌寲杩樺師骞剁鍘伙紝鍘熶綅鐢熸垚楂樹翰鐢垫�?/span>鐨勪簹鐢插熀閱屼腑闂翠綋锛屽疄鐜扮嚎绮掍綋铔嬬櫧璐ㄧ殑鐗瑰紓鎬ф爣璁般�傛渶鍚庯紝浣滆�呴�氳繃閫夋嫨鎬?/span>瀵岄泦鍜屼覆鑱旇川璋卞垎鏋愯鐗瑰紓鎬ф爣璁扮殑绾跨矑浣?/span>铔嬬櫧璐紝瀹炵幇楂?/span>瑕嗙洊鐜?/span>銆侀珮鐗瑰紓鎬т互鍙婃椂绌哄垎杈ㄧ殑绾跨矑浣撹泲鐧借川缁勫鍒嗘瀽銆傜粨鏋滆〃鏄庯紝璇ユ柟娉曞湪HeLa缁嗚優浠ュ強鏇撮毦杞煋鐨?/span>RAW264.7缁嗚優涓兘澶熼壌瀹氬埌瓒呰繃200涓嚎绮掍綋铔嬬櫧璐紝鐗瑰紓鎬ч珮杈?/span>70%銆傛澶栵紝浣滆�呰繕鍒╃敤杩欎竴绛栫暐鍦?/span>鐐庣棁鍒烘縺涓?/span>鐨?/span>RAW264.7缁嗚優涓?/span>瀹炵幇浜嗙嚎绮掍綋铔嬬櫧璐ㄧ粍鐨勫姩鎬佸垎鏋愶紝闄や簡鑳藉楂樼壒寮傛�у湴閴村畾绾跨矑浣撹泲鐧借川浠ュ杩樿兘澶熷瀹冧滑鍦ㄧ値鐥囧埡婵�鍓嶅悗鐨?/span>涓板害鍙樺寲杩涜鐩戞祴锛?/span>瀹炵幇澶氱淮搴︾殑绾跨矑浣撹泲鐧借川缁勫鍒嗘瀽銆?/span>
鍥?/span>2. 绾跨矑浣撹泲鐧借川缁勭殑涓茶仈璐ㄨ氨鍒嗘瀽缁撴灉
缁间笂锛?/span>璇ラ」鐮旂┒寮�鍙戜簡鍩轰簬鍏夊偓鍖栫殑鐢熺墿姝ｄ氦鍓垏鍙嶅簲浠ュ強閭昏繎鏍囪鐨勭嚎绮掍綋铔嬬櫧璐ㄧ粍瀛︽妧鏈?/span>CAT-Prox銆傝鎶�鏈?/span>鍦ㄤ笉闇�瑕佽川绮掕浆鏌撶殑鎯呭喌涓嬪疄鐜颁簡鏃剁┖鍒嗚鲸鐨勭嚎绮掍綋铔?/span>鐧借川缁勫鍒嗘瀽锛屼负鏃ュ悗鍦ㄦ椿浣撲腑杩涗竴姝ョ爺绌朵簹缁嗚優鍖哄煙鐨勫姩鎬佽泲鐧借川缁勬墦涓嬩簡鍧氬疄鐨勫熀纭�锛屼粠鑰岃兘澶熸洿娣卞叆鏇寸粏鑷村湴瀵圭敓鐗╁杩囩▼杩涜瑙ｆ瀽銆傝鐮旂┒鎴愭灉杩戞棩浠?/span>鈥?/span>Bioorthogonal Photocatalytic Decaging-Enabled Mitochondrial Proteomics鈥濅负棰樺彂琛ㄤ簬Journal of the American Chemical Society (//doi.org/10.1021/jacs.1c09171)銆?/span>
鍖椾含澶у鍖栧涓庡垎瀛愬伐绋嬪闄㈠崥澹爺绌剁敓榛勫畻鐓溿�佸垬瀛愮惁涓鸿璁烘枃鐨勫叡鍚岀涓�浣滆�咃紝鍖椾含澶у鍖栧涓庡垎瀛愬伐绋嬪闄€�佸寳澶?/span>-娓呭崕鐢熷懡绉戝鑱斿悎涓績闄堥箯鏁欐巿鍜屽寳浜ぇ瀛﹀寲瀛︿笌鍒嗗瓙宸ョ▼瀛﹂櫌鐨勬▕鏂板厓鍓爺绌跺憳涓鸿璁烘枃鐨勫叡鍚岄�氳浣滆�呫�傝宸ヤ綔寰楀埌浜嗗浗瀹惰嚜鐒剁瀛﹀熀閲戝銆佺鎶�閮ㄣ�佸寳浜垎瀛愮瀛﹀浗瀹剁爺绌朵腑蹇冧互鍙婂寳澶?/span>-娓呭崕鐢熷懡绉戝鑱斿悎涓績鐨勮祫鍔┿�?/span>
鍘熸枃閾炬帴锛?/span>//pubs.acs.org/doi/10.1021/jacs.1c09171