瀹為獙瀹ゆ柊闂?/h4>
浣曞窛/璐炬鑺宠棰樼粍鍦ˋngew. Chem. Int. Ed. 鎶ラ亾RNA淇グm6A鍗曞熀鍥犲崟纰卞熀妫�娴嬫柊鎶�鏈?/h1>
浣滆�咃細
鏉ユ簮锛?nbsp;
鍙戝竷鏃ユ湡:2018-10-25
2018骞?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">10鏈堬紝鍖椾含澶у鍖栧涓庡垎瀛愬伐绋嬪闄綍宸?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">/璐炬鑺宠棰樼粍鍦ㄣ�?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">Angew. Chem. Int. Ed. 锛堝痉鍥藉簲鐢ㄥ寲瀛︼級銆嬫潅蹇楀湪绾垮彂琛ㄩ涓衡�?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">An Elongation and Ligation-Based qPCR Amplification Method for the Radiolabeling-Free Detection of Locus-Specific N6-methyladenosine Modifications鈥?/span>鐨勭爺绌惰鏂?span> (DOI:10.1002/anie.201807942)銆傛枃绔犳姤閬撲簡涓�绉嶅揩鎹锋娴?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">RNA涓?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">N6-鐢插熀鑵哄槍鍛わ紙N6-methyladenosine锛?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">m6A锛夌殑鏂版柟娉曘�?/p>
琛ㄨ杞綍缁勫锛?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">epitranscriptomics锛屽張绉扳�?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">RNA琛ㄨ閬椾紶瀛︹�濓級鏄繎骞存潵鍏磋捣鐨勫墠娌跨鐮旈鍩熴�?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">RNA鍖栧淇グm6A浣滀负琛ㄨ杞綍缁勫涓殑鏄庢槦淇グ锛屾槸鐪熸牳mRNA鍜岄潪缂栫爜RNA涓殑涓昏鍖栧淇グ銆?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">m6A鍙互琚慨楗伴叾鍜屽幓淇グ閰惰繘琛屽姩鎬佸彲閫嗚皟鑺傦紝骞惰缁撳悎铔嬬櫧璇嗗埆璋冩帶RNA鍔犲伐浠ｈ阿锛屽弬涓庤澶氶噸瑕佺敓鐗╁璋冭妭锛屽鍛ㄦ湡鑺傚緥銆佸共缁嗚優鍒嗗寲銆佺檶鐥囧彂鐢熷彂灞曞強RNA鐥呮瘨绛夈�?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">m6A妫�娴嬪浜庡叾鍒嗗瓙鐢熺墿瀛﹀姛鑳藉拰鐩稿叧鐤剧梾鐮旂┒闈炲父閲嶈銆傜洰鍓嶆姤閬撶殑妫�娴嬫柟娉曚腑鍙湁SCARLET鏂规硶鑳藉瀹炵幇鍗?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">mRNA鍜?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">lncRNA涓?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">m6A鍗曠⒈鍩哄垎杈ㄧ巼鐨勬娴嬶紝浣嗚鏂规硶鎿嶄綔澶嶆潅鑰楁椂锛岄渶瑕佽緝澶у墏閲忕殑鏀惧皠鎬у悓浣嶇礌鏍囪锛岄毦浠ュ箍娉涘簲鐢ㄣ�?/p>
鍖椾含澶у鍖栧瀛﹂櫌浣曞窛/璐炬鑺宠棰樼粍寮�鍙戜簡SELECT鏂规硶锛屽師鐞嗗鍥?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">1鎵�绀猴紝璇ユ柟娉曠畝鍗曞揩鎹凤紝鐢ㄦ椂鍙渶涓夊皬鏃讹紝鑳藉瀹炵幇浣庝赴搴﹁浆褰曟湰涓?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">m6A鍗曠⒈鍩哄垎杈ㄧ巼鐨勬娴嬨�傝鏂规硶鍩轰簬m6A淇グ鐨勪袱鐐圭壒鎬э細锛?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">i锛夎兘澶熼樆纰?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">DNA鑱氬悎閰跺湪鍙嶈浆褰曡繃绋嬬殑鍗曠⒈鍩哄欢浼革紱锛?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">ii锛夎兘澶熼檷浣庣己鍙ｈ繛鎺ラ叾鐨勮繛鎺ユ晥鐜囥�?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">SELECT鏂规硶閲囩敤鑽у厜瀹氶噺PCR鐨勬柟娉曡繘琛屽畾閲忋�傜粨鍚堟柟娉曚紭鍖栵紝鍙戝睍浜?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">SELECT鏂规硶鐨?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">3涓簲鐢ㄥ疄渚嬶細锛?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">i锛夊湪鐢熺墿瀛︽牱鏈�?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">RNA鎴栨�?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">mRNA涓娴?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">mRNA鎴?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">lncRNA涓婂崟浣嶇偣鏄惁鍚湁m6A淇グ锛涳紙ii锛夋娴嬬敓鐗╁鏍锋湰涓壒瀹?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">m6A浣嶇偣鐨勪慨楗版瘮渚嬶紱锛?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">iii锛夐壌瀹?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">m6A鐢插熀杞Щ閰舵垨鍘荤敳鍩洪叾鐨勭敓鐞嗕綔鐢ㄩ澏鐐广�?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">SELECT鏂规硶涓嶄粎绠�鍖栦簡鐢熺墿瀛︽牱鍝佷腑m6A淇グ鐨勬娴嬭繃绋嬶紝瀹炵幇浣庝赴搴?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">RNA涓?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">m6A鍗曠⒈鍩哄垎杈ㄧ巼鐨勬娴嬶紝杩樿兘澶熷簲鐢ㄤ簬鍏朵粬RNA鍖栧淇グ鐨勬娴嬩腑锛屼緥濡?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">N1-鐢插熀鑵哄槍鍛わ紙N1-methyladenosine锛?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">m1A锛夊拰2鈥?O-鐢插熀鍖栦慨楗帮紙2鈥?O-methylation锛?span style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; font-family: Calibri, serif; padding-top: 0px; -webkit-font-smoothing: subpixel-antialiased">Nm)绛夈�?/p>
鍥?/span>1. SELECT鏂规硶鍘熺悊绀烘剰鍥?/span>
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