瀹為獙瀹ゆ柊闂?/h4>
闄堝叴璇鹃缁勬彮绀哄啗鍥㈣弻鏉ユ簮鐨勬柊鍨婳-绯栧熀杞Щ閰舵満鐞?/h1>
浣滆�咃細
鏉ユ簮锛?nbsp;
鍙戝竷鏃ユ湡:2019-01-08
2019骞?/span>1鏈?/span>8鏃ワ紝鍖椾含澶у鍖栧涓庡垎瀛愬伐绋嬪闄€�?/span>鍖楀ぇ-娓呭崕鐢熷懡绉戝鑱斿悎涓績闄堝叴鏁欐巿璇鹃缁勫湪Nature Chemical Biology鏉傚織鍦ㄧ嚎鍙戣〃浜嗛涓?/span>鈥?/span>Legionella effector SetA as a general O-glucosyltransferase for eukaryotic proteins鈥?/span>锛?/span>DOI: 10.1038/s41589-018-0189-y锛?/span>鐨勭爺绌惰鏂?/span>锛?/span>鎶ラ亾閫氳繃鍖栧閰舵硶鏍囪绛栫暐锛屽疄鐜颁簡瀵瑰棞鑲哄啗鍥㈣弻鏁堝簲铔嬬櫧绯栧熀杞Щ閰?/span>SetA鐨勮泲鐧藉簳鐗╅壌瀹氾紝骞堕槓鏄?/span>浜?/span>SetA鐨?/span>搴曠墿閫夋嫨鏈虹悊锛?/span>涓虹硸铔嬬櫧鐨勪汉宸ュ悎鎴愭彁渚涗簡涓�绉?/span>鏈夊姏鐨勫伐鍏?/span>銆?/span>
绯栧熀鍖栦慨楗版槸涓�绉嶅箍娉涘瓨鍦ㄤ笖閲嶈鐨勮泲鐧借川缈昏瘧鍚庝慨楗?/span>锛?/span>鐢辩硸鍩鸿浆绉婚叾鍌寲褰㈡垚銆?/span>鎶椾綋銆佺柅鑻?/span>绛?/span>璁稿涓村簥娌荤枟鎬?/span>铔嬬櫧璐?/span>閮藉叿鏈?/span>鐗瑰紓鎬?/span>绯栧熀鍖栦慨楗?/span>锛?/span>涓?/span>瀵?/span>铔嬬櫧娲绘�?/span>鑷冲叧閲嶈銆?/span>鍩轰簬绯栧熀杞Щ閰?/span>鐨?/span>鍖栧閰舵硶鍚堟垚鏂规硶锛?/span>鏄幏寰楀潎涓�绯栬泲鐧界殑鏈夋晥鎵嬫涔嬩竴銆傚洜姝わ紝鎵惧鎬ц川浼樿壇鐨勫悇绉嶇硸鍩鸿浆绉婚叾鍗佸垎鍏抽敭銆傚姣旂湡鏍哥粏鑳烇紝缁嗚弻鏉ユ簮鐨勭硸鍩鸿浆绉婚叾鍏锋湁鏄撹〃杈剧函鍖栧拰鍌寲浜х巼楂樼瓑浼樿壇鎬ц川锛屽父琚敼閫犱负浜哄伐鍚堟垚绯栬泲鐧界殑宸ュ叿閰躲�傜劧鑰岋紝铔嬬櫧O-杩炴帴绯栧熀鍖栦慨楗板湪鍘熸牳鐢熺墿涓庣湡鏍哥敓鐗╀箣闂村苟涓嶄繚瀹堬紝杩欐垚涓轰簡鍒╃敤缁嗚弻绯栧熀鍖栫郴缁熺敓浜х湡鏍哥敓鐗?/span>O-绯栬泲鐧界殑涓�澶х摱棰堛�備负浜嗚В鍐宠繖涓棶棰橈紝闄堝叴鏁欐巿璇鹃缁?/span>瀵圭梾鍘熻弻-瀹夸富鐩镐簰浣滅敤涓殑铔嬬櫧璐ㄧ硸鍩哄寲杩涜浜嗘帰绱€�?/span>
鏌愪簺鐥呭師鑿屽叆渚靛涓?/span>缁嗚優鍚庯紝浼?/span>鍒嗘硨鍏锋湁绯栧熀杞Щ閰舵椿鎬х殑鏁堝簲铔嬬櫧銆傝繖浜涙晥搴旇泲鐧藉線寰�鍒╃敤瀹夸富鐨勭硸渚涗綋锛?/span>骞朵笖淇グ瀹夸富鐨勯澏铔嬬櫧銆?/span>鍥犳锛屽杩欑被绯栧熀杞Щ閰剁殑宸ョ▼鍖栵紝鍙负瀹炵幇鐪熸牳鐢熺墿O-绯栬泲鐧界殑鍚堟垚鎻愪緵鏂扮殑鏈夋晥绛栫暐銆?/span>鍡滆偤鍐涘洟鑿?/span>鏁堝簲铔嬬櫧SetA鍏锋湁O-钁¤悇绯栬浆绉婚叾娲绘�э紝鐒惰�屽叾搴曠墿铔嬬櫧灏氭湭琚壌瀹氥�?/span>闄堝叴璇鹃缁?/span>棣栧厛寮�鍙戜簡鑳藉瀹炵幇鐗瑰紓鎬?/span>鏍囪鍜?/span>閫夋嫨鎬?/span>瀵岄泦鐨勫寲瀛﹂叾娉?/span>绛栫暐銆?/span>浠栦滑璁捐琚?/span>SetA鍒╃敤鐨?/span>鐢熺墿姝ｄ氦鍩哄洟淇グ鐨勭硸渚涗綋锛?/span>灏胯嫹浜岀７閰?/span>-6-鍙犳爱-钁¤悇绯栵紝UDP-6AzGlc锛夛紝灏嗗彔姘憽钀勭硸6AzGlc杞Щ鑷冲簳鐗╄泲鐧姐�?/span>閫氳繃鐐瑰嚮鍖栧鍙嶅簲锛屼笌鍙垏鍓茬敓鐗╃礌鎺㈤拡杩炴帴锛?/span>瀹炵幇瀵?/span>搴曠墿铔嬬櫧鐨?/span>閫夋嫨鎬у瘜闆嗗拰浣嶇偣鐗瑰紓鎬?/span>璐ㄨ氨閴村畾(涓嬪浘)銆?/span>璐ㄨ氨鍒嗘瀽閴村畾鍒?/span>鍒嗗竷浜?/span>276涓泲鐧界殑317涓?/span>O-glucose淇グ浣嶇偣銆?/span>澶╃劧绯栦緵浣撴爣璁?/span>鍙婂啗鍥㈣弻渚垫煋瀹為獙锛?/span>楠岃瘉浜?/span>SetA鍙互淇グ浼楀鐨?/span>搴曠墿铔嬬櫧銆?/span>
鍖栧閰舵硶鏍囪绛栫暐娴佺▼绀烘剰鍥?/span>
杩涗竴姝ョ爺绌?/span>鍙戠幇琚慨楗拌泲鐧?/span>鍏锋湁S/T#-X-L-P/G (#涓轰慨楗版皑鍩洪吀)搴忓垪淇濆畧鎬?/span>銆?/span>鏍稿績鍌寲缁撴瀯鍩熺殑缁撴瀯妯″瀷琛ㄩ潰锛?/span>绯栦緵浣撶粨鍚?/span>鍙ｈ閭昏繎澶勭殑鐤忔按鍖哄煙鍙備笌浜?/span>SetA鐨勫簳鐗╅�夋嫨銆?/span>闅忓悗锛岀爺绌朵汉鍛樺皢SetA閫夋嫨鎬ц瘑鍒殑S/T-X-L-P/G淇濆畧鎬у簭鍒楋紝寮�鍙戜负O-glucosylation 鏍囩 (GlcTag)锛?/span>瀹炵幇浜嗙湡鏍哥敓鐗╄泲鐧界殑浣嶇偣鐗瑰紓鎬?/span>O-绯?/span>鍩哄寲淇グ(涓嬪浘)銆?/span>
SetA璇嗗埆S/T-X-L-P/G淇濆畧搴忓垪
缁间笂鎵�杩帮紝杩欓」鐮旂┒寮�鍙戜簡鍖栧閰舵硶鏍囪绛栫暐锛?/span>瀹炵幇浜嗗鍐涘洟鑿屾晥搴旇泲鐧界硸鍩鸿浆绉婚叾SetA鐨勫簳鐗╄泲鐧介壌瀹氾紝鎻ず浜?/span>SetA鐨勫簳鐗╀慨楗板叿鏈?/span>S/T-X-L-P/G搴忓垪閫夋嫨鎬?/span>銆?/span>棣栨鍙戠幇浜嗗師鏍哥敓鐗╀腑骞胯氨O-绯栧熀鍖栫郴缁?/span>锛屼负绯栬泲鐧界殑浜哄伐鍚堟垚鎻愪緵浜嗘湁鍔涚殑宸ュ叿銆傝鍖栧閰舵硶鏍囪瀵岄泦绛栫暐鍙?/span>鎺ㄥ箍搴旂敤鍒板绉嶇硸鍩鸿浆绉婚叾鐨勫簳鐗╄泲鐧介壌瀹氾紝淇冭繘鏇村鐨勭硸鍚堟垚宸ュ叿閰?/span>鐨勫紑鍙?/span>锛岃繘鑰?/span>瀹炵幇鍚勭绯?/span>铔嬬櫧鐨?/span>绮惧噯鍚堟垚銆?/span>
鍖楀ぇ-娓呭崕鐢熷懡绉戝鑱斿悎涓績鍗氬＋鐮旂┒鐢?/span>楂樼幉涓?/span>鏈鏂?/span>绗竴浣滆�呫�?/span>闄堝叴鏁欐巿閫氳鑱旂郴浣滆�呫�?/span>鍖楀ぇ鍖栧闄?/span>鏉ラ瞾鍗庢暀鎺堬紝鍖椾含鐢熷懡绉戝鐮旂┒鎵�閭靛嘲鐮旂┒鍛?/span>鍜?/span>鍖楀ぇ鐢熺闄㈣倴淇婂畤鐮旂┒鍛樺弬涓?/span>浜?/span>璇ョ爺绌躲�?/span>璇ョ爺绌跺伐浣滃緱鍒颁簡鍥藉鑷劧绉戝鍩洪噾濮斻�佺鎶�閮?/span>銆?/span>鍖楀ぇ-娓呭崕鐢熷懡绉戝鑱斿悎涓績鐨勮祫鍔┿�?/span>