瀹為獙瀹ゆ柊闂?/h4>
闄堥箯璇鹃缁勫彂灞曠粏鑳炰簰浣滅殑鍘熶綅鎹曡幏鎶�鏈?/h1>
浣滆�咃細
鏉ユ簮锛?nbsp;
鍙戝竷鏃ユ湡:2019-02-18
杩戞棩锛?/span>鍖椾含澶у鍖栧涓庡垎瀛愬伐绋嬪闄€�?/span>鍖楀ぇ-娓呭崕鐢熷懡绉戝鑱斿悎涓績闄堥箯鏁欐巿璇鹃缁?/span>浠モ�?/span>Enzyme-Mediated Intercellular Proximity Labeling for Detecting Cell鈥揅ell Interactions鈥?/span>涓洪鍦?/span>J. Am. Chem. Soc.鏉傚織鎶ラ亾浜嗕竴绉嶈兘澶熷湪娲讳綋鍜?/span>鍘熶綅鎯呭喌涓?/span>鎹曡幏缁嗚優涔嬮棿鐩镐簰浣滅敤鐨勬妧鏈?/span>锛?/span>缇庡浗鍖栧浼?/span>C&E NEWS浜?/span>2鏈?/span>12鏃ヤ互鈥?/span>New labeling method can reveal cell-cell interactions鈥?/span>涓洪杩涜浜嗕笓棰樻姤閬撱�?/span>
涓嶅悓缁嗚優涔嬮棿鐨勭浉浜掍綔鐢ㄥ拰淇℃伅浼犻�掑湪鍖呮嫭鍏嶇柅鍝嶅簲銆?/span>鍣ㄥ畼鍙戣偛銆佺缁忎紶瀵?/span>鍦ㄥ唴鐨勪紬澶氱敓鍛芥椿鍔ㄤ腑閮芥壆婕?/span>鐫�鍏抽敭瑙掕壊銆傜洰鍓嶏紝鐮旂┒缁嗚優浜掍綔鐨勬柟娉?/span>澶?/span>閮?/span>闇�瑕?/span>宸茬煡鍙備笌鐨?/span>缁嗚優绫诲瀷锛岄毦浠ュ湪澶嶆潅鐨勬椿浣撶幆澧冧笅鍙戠幇鎴栫爺绌舵湭鐭ョ殑缁嗚優闂?/span>鐩镐簰浣滅敤銆?/span>涓轰簡瑙ｅ喅杩欎竴闅鹃锛岄檲楣忚棰樼粍鍊熷姪鈥滃畾鍚戣繘鍖栤�濇妧鏈拰鈥?/span>閭昏繎鏍囪鈥濈瓥鐣ワ紝瀹炵幇浜?/span>缁嗚優涔嬮棿鍔ㄦ�佺浉浜掍綔鐢ㄧ殑鍘熶綅鎹曡幏銆傝繖涓�琚懡鍚嶄负EXCELL锛?/span>Enzyme-mediated proximal cell labeling锛?/span>鐨勬妧鏈紝閫氳繃灏?/span>浣滆�?/span>瀹氬悜杩涘寲寰楀埌鐨勫垎閫夐叾锛?/span>mgSrtA锛?/span>灞曠ず鍦?/span>寰呯爺绌剁殑缁嗚優琛ㄩ潰锛?/span>鑳藉瀵逛笌鍏?/span>鐩镐簰浣滅敤鐨?/span>缁嗚優杩涜鍘熶綅鎹曡幏涓庨壌瀹?/span>锛屼负鐮旂┒缁嗚優-缁嗚優鐩?/span>浜掍綔鐢ㄦ彁渚涗簡鏈夊姏鐨勫伐鍏?/span>銆?/span>
鍥?/span> 1 EXCELL鏂规硶鐢ㄤ簬鎹曡幏鍜岄壌瀹?/span>缁嗚優闂寸浉浜掍綔鐢?/span>
婧愯嚜閲戦粍鑹茶憽钀勭悆鑿?/span>鐨?/span>鍒嗛�夐叾Sortase A 锛?/span>SrtA锛?/span>鑳藉鍌寲鍒嗛�夎偨锛?/span>LPETG锛?/span>鍜屽鑱氱敇姘ㄩ吀鐨?/span>鍏变环杩炴帴銆傝繎鏈熸湁鏂囩尞鎶ラ亾灏?/span>SrtA鐢ㄤ簬鐩戞祴灏忛紶浣撳唴鐗瑰畾鍙椾綋-閰嶄綋浠嬪鐨勫厤鐤?/span>缁嗚優浜掍綔杩囩▼1銆備絾鏄鏂规硶闇�瑕佸皢SrtA涓?/span>瀵¤仛鐢樻皑閰稿垎鍒瀺鍚堝湪鐩镐簰浣滅敤鐨?/span>閰嶄綋鍜屽彈浣?/span>缁嗚優涓婏紝鍥犳闇�瑕侀鐭?/span>鐩镐簰浣滅敤鐨?/span>缁嗚優绫诲瀷锛屽苟瀵逛袱绫荤粏鑳?/span>鍚屾椂杩涜鍩哄洜鏀归�?/span>锛屽洜姝ゆ棤娉曟崟鎹夋湭鐭ョ殑缁嗚優闂寸浉浜掍綔鐢?/span>銆?/span>涓轰簡绐佺牬杩欎竴鐡堕锛?/span>闄堥箯璇鹃缁?/span>璁炬兂鑾峰緱涓�绉嶉拡瀵规湭鐭ョ粏鑳炵殑鏍囪鏂规硶銆傜敱浜?/span>缁嗚優琛ㄩ潰铔嬬櫧涓湁澶х害5锛呭惈鏈?/span>N绔崟涓�鐢樻皑閰?/span>锛屽彈姝?/span>鍚彂锛?/span>浠栦滑甯屾湜鑾峰緱涓�绉嶅鍗曚釜鐢樻皑閰哥殑N绔皑鍩鸿繘琛屾爣璁扮殑鍒嗛�夐叾锛?/span>浠庤�岃兘澶熷浠绘剰缁嗚優鍔犱互鏍囪銆備负姝?/span>浠栦滑寤虹珛浜?/span>瀵?/span>SrtA杩涜瀹氬悜杩涘寲鐨勯珮閫氶噺鑽у厜绛涢�?/span>骞冲彴锛屽苟鎴愬姛鑾峰緱浜?/span>瀵瑰崟涓�鐢樻皑閰歌繘琛屾爣璁扮殑楂樻椿鎬?/span>SrtA绐佸彉浣?/span>-mgSrtA锛?/span>鑳藉瀹炵幇瀵?/span>浠绘剰缁嗚優绫诲瀷鐨勬湁鏁堟爣璁般�?/span>
浣滆�呭垎鍒�熷姪浜插拰浣?/span>-鎶楀師锛?/span>ZHER涓?/span>Her2锛夊拰閰嶄綋-鍙椾綋锛?/span>CTLA4涓?/span>B7, CD40L涓?/span>CD40锛夌殑鐩镐簰浣滅敤鏉?/span>楠岃瘉璇ユ柟娉?/span>鐨勫彲闈犳�?/span>銆?/span>瀹為獙璇佹槑灞曠ず鍦ㄧ粏鑳炶〃闈㈢殑mgSrtA鍙?/span>鍊熷姪閭昏繎鏁堝簲锛?/span>灏嗗惈鏈?/span> biotin鏍囩鐨勫垎閫夎偨biotin-LPETG鍏变环鏍囪鍦?/span>涓庡叾鐩镐簰浣滅敤鐨?/span>缁嗚優琛ㄩ潰銆傞�氳繃娴佸紡缁嗚優鏈?/span>妫�娴?/span>锛?/span>鑳藉鍙戠幇biotin鏍囪淇″彿浠呭湪鐩镐簰浣滅敤缁嗚優涓婃湁鏄捐憲鎬у寮猴紝鐢辨鍙?/span>鎴愬姛鍖哄垎鍜屾崟鑾峰彂鐢熺浉浜掍綔鐢ㄧ殑缁嗚優銆?/span>
鍥?/span> 2鍦ㄤ袱绫荤粏鑳炵浉浜掍綔鐢ㄦā鍨嬩笂搴旂敤EXCELL锛屽彲瀹炵幇瀵逛簰浣?/span>缁嗚優鐨勬湁鏁堟爣璁?/span>
璇ユ柟娉曢娆″皢SrtA鏀归�犳垚楂樻椿鎬т笖鍏锋湁鏇?/span>瀹芥硾搴曠墿璋?/span>鐨?/span>鈥?/span>閭昏繎鏍囪鈥濋叾锛屽苟楠岃瘉鍏跺缁嗚優鐩镐簰浣滅敤鐨勬爣璁板拰杩借釜銆?/span>鏂囦腑鎸囧嚭锛?/span>EXCELL鏂规硶鏃犻渶灏嗗鑱氱敇姘ㄩ吀浜嬪厛寮曞叆鍒板璞＄粏鑳炶〃闈紝鍥犳閫傜敤浜庣爺绌?/span>澶嶆潅浣撶郴涓?/span>鏈煡缁嗚優绫诲瀷鐨?/span>鐩镐簰浣滅敤銆?/span>鍚屾椂锛岄壌浜?/span>SrtA搴曠墿鍒嗛�夎偨浠?/span>鍚?/span>浜斾釜姘ㄥ熀閰革紝骞跺凡鎴愬姛搴旂敤浜庡皬榧犱綋鍐?/span>銆傝鏍囪鏂规硶涓嶄粎鏄撲簬鐩戞祴锛屽湪鏈潵杩樺彲浠ヤ笌澶氱涓嬫父鍒嗘瀽骞冲彴鍏煎锛屽畬鎴愬鐩镐簰浣滅敤缁嗚優鐨勬崟鑾枫�佸瘜闆嗗拰娴嬪簭锛?/span>浠庤�岃繘涓�姝ュ緱鍑虹粏鑳為棿鐩镐簰浣滅敤鍦ㄦ椂闂淬�佺┖闂淬�?/span>绫诲瀷绛夊鏂归潰鐨勪俊鎭?/span>銆?/span>
杩戝勾鏉ワ紝涓�绯诲垪铔嬬櫧璐ㄩ偦杩戞爣璁版妧鏈寮�鍙戝苟搴旂敤浜?/span>铔嬬櫧璐?/span>缁勫鐮旂┒, 姝ゆ寮�鍙戠殑EXCELL鎶�鏈?/span>瀹炵幇浜?/span>缁嗚優浜掍綔鐨勫師浣嶆爣璁颁笌鎹?/span>鑾?/span>锛屽皢閭昏繎鏍囪绛栫暐浠庘�滆優鍐呪�?/span>鎷撳睍鑷?/span>鈥滆優澶栤�濈┖闂?/span>锛?/span>灏嗗湪鍏嶇柅搴旂瓟銆佺缁忎紶瀵肩瓑棰嗗煙鍏锋湁骞块様鐨?/span>搴旂敤鍓嶆櫙銆?/span>闄堥箯鏁欐巿涓鸿璁?/span>鏂囩殑閫氳浣滆�?/span>锛?/span>璇鹃缁勫崥澹瘯涓氱敓钁涢煹銆侀檲榫欎负鍏卞悓绗竴浣滆�呫�傝宸ヤ綔寰楀埌浜嗗浗瀹惰嚜鐒?/span>绉戝鍩洪噾濮斻�佺鎶�閮ㄥ拰鍖椾含澶у鍚堟垚涓庡姛鑳界敓鐗╁垎瀛愪腑蹇?/span>鐨?/span>璧勫姪銆?/span>
鍘熸枃閾炬帴锛?/span>
//pubs.acs.org.ccindex.cn/doi/10.1021/jacs.8b10286
C&E NEWS閾炬帴锛?/span>
//cen.acs.org/biological-chemistry/biochemistry/New-labeling-method-reveal-cell/97/web/2019/02