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鍗曠粏鑳?/span>杞綍缁?/span>娴嬪簭鐩墠宸叉垚涓虹敓鐗╁鐮旂┒涓殑鏍稿績鎶�鏈墜娈?/span>锛屽叾鑳藉甯姪鐮旂┒浜哄憳鑾峰緱缁勭粐鏍峰搧涓崟缁嗚優姘村钩鐨勫熀鍥犺〃杈句俊鎭�傜劧鑰岋紝浼犵粺鐨勫崟缁嗚優娴嬪簭娴佺▼闇�瑕?/span>鍏?/span>灏嗙粍缁囨秷鍖栦负鍗曠粏鑳炴偓娑诧紝杩欎竴杩囩▼浼氶�犳垚缁嗚優绌洪棿浣嶇疆淇℃伅鐨勪涪澶便�?/span>宸叉湁鐨勭┖闂?/span>鍒嗚鲸鍗曠粏鑳炶浆褰曠粍鍘熶綅娴嬪簭鎶�鏈線寰�鍙楅檺浜?/span>浣?/span>绌洪棿鍒嗚鲸鐜?/span>銆佷綆閫氶噺鍜岄珮鑳屾櫙绛夐棶棰樸�?/span>
杩戞棩锛屽寳浜ぇ瀛﹀寲瀛︿笌鍒嗗瓙宸ョ▼瀛﹂櫌闄堝叴璇鹃缁勩�侀粍宀╄皧璇鹃缁勪笌鍖椾含澶у绗竴鍖婚櫌鏉ㄨ帀璇鹃缁勫悎浣滃紑鍙戜簡涓�绉嶅叏鏂扮殑绌洪棿鍒嗚鲸鍗曠粏鑳炴祴搴忔妧鏈?/span>锛圤pTAG-seq锛夈�?/span>璇?/span>鏂规硶鍒╃敤鍏夋縺娲荤殑浜氱敳鍩鸿嫰閱屾帰閽?/span>瀵圭壒瀹氫綅缃殑缁嗚優杩涜鑽у厜鏍囪锛?/span>闅忓悗灏嗚鏍囪鐨勭粏鑳炶繘琛屽垎绂诲拰鍗曠粏鑳?/span>浜屼唬寤哄簱娴嬪簭锛?/span>鍦ㄨ幏寰楅珮璐ㄩ噺鍗曠粏鑳炴祴搴忔暟鎹殑鍚屾椂淇濈暀浜?/span>缁嗚優鐨?/span>绌洪棿浣嶇疆淇℃伅銆?/span>
涓轰簡瀹炵幇楂樼┖闂村垎杈ㄧ巼鐨?/span>缁嗚優鏍囪锛?/span>浣滆�?/span>璁捐骞跺悎鎴愪簡鍏夋縺娲绘帰閽圤NPF锛屽苟鍩轰簬鍏跺紑鍙戜簡鍏夋帶缁嗚優鏍囪绛栫暐OpTAG銆傚湪绱鍏?/span>鐓у皠涓嬶紝ONPF杞寲鐢熸垚鍏锋湁鏋佸己鍙嶅簲娲绘�х殑浜氱敳鍩鸿嫰閱屼腑闂翠綋銆傚湪娲荤粏鑳炶〃闈紝璇?/span>涓棿浣撹兘蹇�熷湴涓庣粏鑳炶啘涓婄殑铔嬬櫧璐ㄧ瓑浜叉牳璇曞墏杩涜鍏变环杩炴帴銆?/span>浣滆�?/span>鍦ㄦ帰閽堜笂寮曞叆鐢熺墿绱犳姤鍛婂熀鍥紝骞?/span>鍒╃敤鑽у厜淇グ鐨?/span>閾鹃湁浜插拰绱犲鏍囪杩涜琛ㄥ緛銆?/span>浣滆�?/span>璇佹槑杩欎竴鎺㈤拡鑳藉鍦ㄦ椿缁嗚優琛ㄩ潰瀹炵幇寰堥珮绌洪棿鍒嗚鲸鐜囩殑鏍囪銆傞�氳繃澶氳疆鏍囪鍜岄鑹茬粍鍚堬紝OpTAG鏈�澶氬彲浠ュ湪涓�浠芥牱鍝佷腑鍚屾椂瀹炵幇7涓綅缃殑鏍囪锛屼笖鏍囪鐨勭┖闂村垎杈ㄧ巼鍙互杈惧埌鍗曠粏鑳?/span>姘村钩銆?/span>
浣滆�呴殢鍚庡皢OpTAG鏍囪锛屾祦寮忓垎閫変笌鍗曠粏鑳炴祴搴忕粨鍚堬紝鍙戝睍浜嗕竴绉嶆柊鐨勭┖闂?/span>鍒嗚鲸鍗曠粏鑳炴祴搴忔妧鏈疧pTAG-seq銆備綔鑰呭埄鐢∣pTAG-seq鎺㈢┒浜嗙檶鐥囪縼绉昏繃绋嬬殑鍩哄洜琛ㄨ揪璋冩帶銆傚湪浣撳鏋勫缓鐨勭檶缁嗚優杩佺Щ妯″瀷涓紝浣滆�呭垎鍒澶栭儴鍏锋湁楂樿縼绉绘椿鎬у拰鍐呴儴鐨勭檶缁嗚優杩涜OpTAG-seq鍒嗘瀽銆傜粨鏋滄樉绀猴紝杩欎袱閮ㄥ垎缁嗚優鍦ㄩ檷缁村垎鏋愪腑鍏锋湁鏄庢樉鐨勫垎鐣岋紝璇存槑鍏跺熀鍥犺〃杈惧叿鏈夎緝澶у樊寮傘�備綔鑰呴壌瀹氬埌璁稿鍦ㄩ珮杩佺Щ娲绘�х粏鑳炰腑涓婅皟鎴栦笅璋冪殑鍩哄洜銆傚叾涓紝涓婅皟鐨勮繖浜涘熀鍥犳樉钁楀湴瀵岄泦鍦ㄧ粏鑳炶縼绉诲拰缁嗚優杩愬姩绛夊姛鑳芥潯鐩腑銆備綔鑰呭鍏朵腑涓や釜闈舵爣杩涜浜嗗厤鐤崸鍏夐獙璇侊紝鍙戠幇KRT17鏄捐憲鍦板湪澶栭儴缁嗚優楂樿〃杈撅紝鑰孧UC16鍒欏湪鍐呴儴缁嗚優楂樿〃杈撅紝涓?/span>娴嬪簭缁撴灉楂樺害涓�鑷淬�傛澶栵紝浣滆�呰繕鎴愬姛鍒╃敤OpTAG鍦ㄥ皬榧犺偩鑴忓垏鐗囦腑瀵逛笉鍚屽尯鍩熻繘琛岄珮鏁堣崸鍏夋爣璁帮紝璇佹槑杩欎竴鏂规硶鍦ㄧ粍缁囧眰闈篃鍏锋湁骞块様鐨勫簲鐢ㄥ墠鏅�備互涓婄粨鏋滆鏄嶰pTAG-seq鑳藉鏈夋晥鍦板疄鐜扮┖闂?/span>鍒嗚鲸鍗曠粏鑳炶浆褰曠粍娴嬪簭锛屼负鐮旂┒缁嗚優鍜岀粍缁囦腑鍩哄洜琛ㄨ揪鐨勬椂绌鸿皟鎺ф彁渚涗簡鏈夊姏鐨勫寲瀛﹀伐鍏枫�?/span>
璇?/span>宸ヤ綔杩戞棩浠?/span>鈥淥ptical Cell Tagging for Spatially Resolved Single-Cell RNA Sequencing鈥?/span>涓洪鍙戣〃鍦?/span>Angewandte Chemie International Edition銆?/span>鍗氬＋鐢熷攼楹?/span>鍜?/span>鍒樼拹涓?/span>鏂囩珷鍏?/span>鍚岀涓�浣滆�呫�傞儹鎬″叞銆佸紶鏃?/span>銆?/span>寮犵粛鐒?/span>銆?/span>璐剧嚂鍗氬＋銆?/span>鏉滈�搁鍗氬＋銆佹垚娉㈠崥澹瓑涓?/span>璇?/span>宸ヤ綔鍋氬嚭浜嗛噸瑕佽础鐚�?/span>闄堝叴鏁欐巿銆?/span>榛勫博璋?/span>鏁欐巿鍜?/span>鏉ㄨ帀鏁欐巿涓?/span>璇ヨ鏂囩殑鍏卞悓閫氳浣滆�?/span>銆?/span>璇ュ伐浣滃緱鍒颁簡鍥藉鑷劧绉戝鍩洪噾濮斻�佺鎶�閮?/span>銆?/span>鍖椾含鍒嗗瓙绉戝鍥藉鐮旂┒涓績浠ュ強鍖楀ぇ-娓呭崕鐢熷懡绉戝鑱斿悎涓績鐨勮祫鍔┿�?/span>
鍘熸枃閾炬帴锛?/span>//onlinelibrary.wiley.com/doi/10.1002/anie.202113929